Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif

Kuglae Kim, Soon-Kyeong Kwon, Sung-Hoon Jun, Jeong Seok Cha, Hoyoung Kim, Weontae Lee, Jihyun F. Kim () and Hyun-Soo Cho ()
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Kuglae Kim: Yonsei University
Soon-Kyeong Kwon: Yonsei University
Sung-Hoon Jun: Yonsei University
Jeong Seok Cha: Yonsei University
Hoyoung Kim: Yonsei University
Weontae Lee: Yonsei University
Jihyun F. Kim: Yonsei University
Hyun-Soo Cho: Yonsei University

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract A novel light-driven chloride-pumping rhodopsin (ClR) containing an ‘NTQ motif’ in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08T determined under two conditions at 2.0 and 1.56 Å resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a ‘3 omega motif’ formed by three non-consecutive aromatic amino acids that is correlated with the B–C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

Date: 2016
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DOI: 10.1038/ncomms12677

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