Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells

Sabine Fièvre, Mario Carta, Ingrid Chamma, Virginie Labrousse, Olivier Thoumine and Christophe Mulle ()
Additional contact information
Sabine Fièvre: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux
Mario Carta: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux
Ingrid Chamma: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux
Virginie Labrousse: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux
Olivier Thoumine: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux
Christophe Mulle: Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, University of Bordeaux

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract Distinct subtypes of ionotropic glutamate receptors can segregate to specific synaptic inputs in a given neuron. Using functional mapping by focal glutamate uncaging in CA3 pyramidal cells (PCs), we observe that kainate receptors (KARs) are strictly confined to the postsynaptic elements of mossy fibre (mf) synapses and excluded from other glutamatergic inputs and from extrasynaptic compartments. By molecular replacement in organotypic slices from GluK2 knockout mice, we show that the faithful rescue of KAR segregation at mf-CA3 synapses critically depends on the amount of GluK2a cDNA transfected and on a sequence in the GluK2a C-terminal domain responsible for interaction with N-cadherin. Targeted deletion of N-cadherin in CA3 PCs greatly reduces KAR content in thorny excrescences and KAR-EPSCs at mf-CA3 synapses. Hence, multiple mechanisms combine to confine KARs at mf-CA3 synapses, including a stringent control of the amount of GluK2 subunit in CA3 PCs and the recruitment/stabilization of KARs by N-cadherins.

Date: 2016
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms12738 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12738

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms12738

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().