Molecular transport through large-diameter DNA nanopores

Swati Krishnan, Daniela Ziegler, Vera Arnaut, Thomas G. Martin, Korbinian Kapsner, Katharina Henneberg, Andreas R. Bausch, Hendrik Dietz and Friedrich C. Simmel ()
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Swati Krishnan: Technische Universität München
Daniela Ziegler: Technische Universität München
Vera Arnaut: Technische Universität München
Thomas G. Martin: Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München
Korbinian Kapsner: Technische Universität München
Katharina Henneberg: Technische Universität München
Andreas R. Bausch: Technische Universität München
Hendrik Dietz: Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München
Friedrich C. Simmel: Technische Universität München

Nature Communications, 2016, vol. 7, issue 1, 1-7

Abstract: Abstract DNA-based nanopores are synthetic biomolecular membrane pores, whose geometry and chemical functionality can be tuned using the tools of DNA nanotechnology, making them promising molecular devices for applications in single-molecule biosensing and synthetic biology. Here we introduce a large DNA membrane channel with an ≈4 nm diameter pore, which has stable electrical properties and spontaneously inserts into flat lipid bilayer membranes. Membrane incorporation is facilitated by a large number of hydrophobic functionalizations or, alternatively, streptavidin linkages between biotinylated channels and lipids. The channel displays an Ohmic conductance of ≈3 nS, consistent with its size, and allows electrically driven translocation of single-stranded and double-stranded DNA analytes. Using confocal microscopy and a dye influx assay, we demonstrate the spontaneous formation of membrane pores in giant unilamellar vesicles. Pores can be created both in an outside-in and an inside-out configuration.

Date: 2016
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DOI: 10.1038/ncomms12787

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