Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome

Richard S. Beard, Xiaoyuan Yang, Jamie E. Meegan, Jonathan W. Overstreet, Clement G.Y. Yang, John A. Elliott, Jason J. Reynolds, Byeong J. Cha, Christopher D. Pivetti, David A. Mitchell, Mack H. Wu, Robert J. Deschenes and Sarah Y. Yuan ()
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Richard S. Beard: Morsani College of Medicine, University of South Florida
Xiaoyuan Yang: Morsani College of Medicine, University of South Florida
Jamie E. Meegan: Morsani College of Medicine, University of South Florida
Jonathan W. Overstreet: Morsani College of Medicine, University of South Florida
Clement G.Y. Yang: Morsani College of Medicine, University of South Florida
John A. Elliott: Morsani College of Medicine, University of South Florida
Jason J. Reynolds: Morsani College of Medicine, University of South Florida
Byeong J. Cha: Morsani College of Medicine, University of South Florida
Christopher D. Pivetti: School of Medicine, University of California at Davis
David A. Mitchell: Morsani College of Medicine, University of South Florida
Mack H. Wu: Morsani College of Medicine, University of South Florida
Robert J. Deschenes: Morsani College of Medicine, University of South Florida
Sarah Y. Yuan: Morsani College of Medicine, University of South Florida

Nature Communications, 2016, vol. 7, issue 1, 1-19

Abstract: Abstract Endothelial dysfunction is a hallmark of systemic inflammatory response underlying multiple organ failure. Here we report a novel function of DHHC-containing palmitoyl acyltransferases (PATs) in mediating endothelial inflammation. Pharmacological inhibition of PATs attenuates barrier leakage and leucocyte adhesion induced by endothelial junction hyperpermeability and ICAM-1 expression during inflammation. Among 11 DHHCs detected in vascular endothelium, DHHC21 is required for barrier response. Mice with DHHC21 function deficiency (Zdhhc21dep/dep) exhibit marked resistance to injury, characterized by reduced plasma leakage, decreased leucocyte adhesion and ameliorated lung pathology, culminating in improved survival. Endothelial cells from Zdhhc21dep/dep display blunted barrier dysfunction and leucocyte adhesion, whereas leucocytes from these mice did not show altered adhesiveness. Furthermore, inflammation enhances PLCβ1 palmitoylation and signalling activity, effects significantly reduced in Zdhhc21dep/dep and rescued by DHHC21 overexpression. Likewise, overexpression of wild-type, not mutant, PLCβ1 augments barrier dysfunction. Altogether, these data suggest the involvement of DHHC21-mediated PLCβ1 palmitoylation in endothelial inflammation.

Date: 2016
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DOI: 10.1038/ncomms12823

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