A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors

Stefania Averaimo, Ahlem Assali, Oriol Ros, Sandrine Couvet, Yvrick Zagar, Ioana Genescu, Alexandra Rebsam and Xavier Nicol ()
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Stefania Averaimo: Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
Ahlem Assali: Sorbonne Universités, UPMC University Paris 06, UMR_S 839
Oriol Ros: Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
Sandrine Couvet: Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
Yvrick Zagar: Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
Ioana Genescu: Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision
Alexandra Rebsam: Sorbonne Universités, UPMC University Paris 06, UMR_S 839
Xavier Nicol: Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision

Nature Communications, 2016, vol. 7, issue 1, 1-12

Abstract: Abstract The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and are required for shaping the nervous system connectivity in vivo remains controversial. Here, we report a role for lipid rafts in generating domains of local cAMP signalling in axonal growth cones downstream of ephrin-A repulsive guidance cues. Ephrin-A-dependent retraction of retinal ganglion cell axons involves cAMP signalling restricted to the vicinity of lipid rafts and is independent of cAMP modulation outside of this microdomain. cAMP modulation near lipid rafts controls the pruning of ectopic axonal branches of retinal ganglion cells in vivo, a process requiring intact ephrin-A signalling. Together, our findings indicate that lipid rafts structure the subcellular organization of intracellular cAMP signalling shaping axonal arbors during the nervous system development.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12896

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DOI: 10.1038/ncomms12896

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