Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

Ranhua Xiong, Roosmarijn E. Vandenbroucke, Katleen Broos, Toon Brans, Elien Van Wonterghem, Claude Libert, Jo Demeester, Stefaan C. De Smedt and Kevin Braeckmans ()
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Ranhua Xiong: Laboratory of General Biochemistry and Physical Pharmacy, Ghent University
Roosmarijn E. Vandenbroucke: Inflammation Research Center, VIB
Katleen Broos: Anabiotec nv
Toon Brans: Laboratory of General Biochemistry and Physical Pharmacy, Ghent University
Elien Van Wonterghem: Inflammation Research Center, VIB
Claude Libert: Inflammation Research Center, VIB
Jo Demeester: Laboratory of General Biochemistry and Physical Pharmacy, Ghent University
Stefaan C. De Smedt: Laboratory of General Biochemistry and Physical Pharmacy, Ghent University
Kevin Braeckmans: Laboratory of General Biochemistry and Physical Pharmacy, Ghent University

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Sizing nanomaterials in complex biological fluids, such as blood, remains a great challenge in spite of its importance for a wide range of biomedical applications. In drug delivery, for instance, it is essential that aggregation of protein-based drugs is avoided as it may alter their efficacy or elicit immune responses. Similarly it is of interest to determine which size of molecules can pass through biological barriers in vivo to diagnose pathologies, such as sepsis. Here, we report on continuous fluorescence recovery after photobleaching (cFRAP) as a analytical method enabling size distribution measurements of nanomaterials (1–100 nm) in undiluted biological fluids. We demonstrate that cFRAP allows to measure protein aggregation in human serum and to determine the permeability of intestinal and vascular barriers in vivo. cFRAP is a new analytical technique that paves the way towards exciting new applications that benefit from nanomaterial sizing in bio-fluids.

Date: 2016
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DOI: 10.1038/ncomms12982

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