Human acid sphingomyelinase structures provide insight to molecular basis of Niemann–Pick disease

Yan-Feng Zhou (), Matthew C. Metcalf, Scott C. Garman, Tim Edmunds, Huawei Qiu and Ronnie R. Wei ()
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Yan-Feng Zhou: Biologics Research, Sanofi
Matthew C. Metcalf: University of Massachusetts Amherst
Scott C. Garman: University of Massachusetts Amherst
Tim Edmunds: Biologics Research, Sanofi
Huawei Qiu: Biologics Research, Sanofi
Ronnie R. Wei: Biologics Research, Sanofi

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Acid sphingomyelinase (ASM) hydrolyzes sphingomyelin to ceramide and phosphocholine, essential components of myelin in neurons. Genetic alterations in ASM lead to ASM deficiency (ASMD) and have been linked to Niemann–Pick disease types A and B. Olipudase alfa, a recombinant form of human ASM, is being developed as enzyme replacement therapy to treat the non-neurological manifestations of ASMD. Here we present the human ASM holoenzyme and product bound structures encompassing all of the functional domains. The catalytic domain has a metallophosphatase fold, and two zinc ions and one reaction product phosphocholine are identified in a histidine-rich active site. The structures reveal the underlying catalytic mechanism, in which two zinc ions activate a water molecule for nucleophilic attack of the phosphodiester bond. Docking of sphingomyelin provides a model that allows insight into the selectivity of the enzyme and how the ASM domains collaborate to complete hydrolysis. Mapping of known mutations provides a basic understanding on correlations between enzyme dysfunction and phenotypes observed in ASMD patients.

Date: 2016
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DOI: 10.1038/ncomms13082

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