NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

Dawei Yan, Vanathy Easwaran, Vivian Chau, Masanori Okamoto, Matthew Ierullo, Mitsuhiro Kimura, Akira Endo, Ryoichi Yano, Asher Pasha, Yunchen Gong, Yong-Mei Bi, Nicolas Provart, David Guttman, Anne Krapp, Steven J. Rothstein and Eiji Nambara ()
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Dawei Yan: University of Toronto
Vanathy Easwaran: University of Toronto
Vivian Chau: University of Toronto
Masanori Okamoto: Arid Land Research Center, Tottori University
Matthew Ierullo: University of Toronto
Mitsuhiro Kimura: University of Toronto
Akira Endo: University of Toronto
Ryoichi Yano: Faculty of Life and Environmental Sciences, University of Tsukuba
Asher Pasha: University of Toronto
Yunchen Gong: University of Toronto
Yong-Mei Bi: University of Guelph
Nicolas Provart: University of Toronto
David Guttman: University of Toronto
Anne Krapp: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay
Steven J. Rothstein: University of Guelph
Eiji Nambara: University of Toronto

Nature Communications, 2016, vol. 7, issue 1, 1-11

Abstract: Abstract Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

Date: 2016
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DOI: 10.1038/ncomms13179

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