LWD–TCP complex activates the morning gene CCA1 in Arabidopsis

Jing-Fen Wu, Huang-Lung Tsai, Ignasius Joanito, Yi-Chen Wu, Chin-Wen Chang, Yi-Hang Li, Ying Wang, Jong Chan Hong, Jhih-Wei Chu, Chao-Ping Hsu and Shu-Hsing Wu ()
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Jing-Fen Wu: Institute of Plant and Microbial Biology, Academia Sinica,
Huang-Lung Tsai: Institute of Plant and Microbial Biology, Academia Sinica,
Ignasius Joanito: Institute of Chemistry, Academia Sinica
Yi-Chen Wu: Institute of Plant and Microbial Biology, Academia Sinica,
Chin-Wen Chang: Institute of Plant and Microbial Biology, Academia Sinica,
Yi-Hang Li: Institute of Plant and Microbial Biology, Academia Sinica,
Ying Wang: Institute of Plant and Microbial Biology, Academia Sinica,
Jong Chan Hong: Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University
Jhih-Wei Chu: National Chiao Tung University
Chao-Ping Hsu: Institute of Chemistry, Academia Sinica
Shu-Hsing Wu: Institute of Plant and Microbial Biology, Academia Sinica,

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract A double-negative feedback loop formed by the morning genes CIRCADIAN CLOCK ASSOCIATED1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY) and the evening gene TIMING OF CAB EXPRESSION1 (TOC1) contributes to regulation of the circadian clock in Arabidopsis. A 24-h circadian cycle starts with the peak expression of CCA1 at dawn. Although CCA1 is targeted by multiple transcriptional repressors, including PSEUDO-RESPONSE REGULATOR9 (PRR9), PRR7, PRR5 and CCA1 HIKING EXPEDITION (CHE), activators of CCA1 remain elusive. Here we use mathematical modelling to infer a co-activator role for LIGHT-REGULATED WD1 (LWD1) in CCA1 expression. We show that the TEOSINTE BRANCHED 1-CYCLOIDEA-PCF20 (TCP20) and TCP22 proteins act as LWD-interacting transcriptional activators. The concomitant binding of LWD1 and TCP20/TCP22 to the TCP-binding site in the CCA1 promoter activates CCA1. Our study reveals activators of the morning gene CCA1 and provides an action mechanism that ensures elevated expression of CCA1 at dawn to sustain a robust clock.

Date: 2016
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DOI: 10.1038/ncomms13181

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