A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Patrick M. Shih, Khanh Vuu, Nasim Mansoori, Leïla Ayad, Katherine B. Louie, Benjamin P. Bowen, Trent R. Northen and Dominique Loqué ()
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Patrick M. Shih: Joint BioEnergy Institute, Emery Station East
Khanh Vuu: Joint BioEnergy Institute, Emery Station East
Nasim Mansoori: Joint BioEnergy Institute, Emery Station East
Leïla Ayad: Joint BioEnergy Institute, Emery Station East
Katherine B. Louie: Lawrence Berkeley National Laboratory
Benjamin P. Bowen: Lawrence Berkeley National Laboratory
Trent R. Northen: Joint BioEnergy Institute, Emery Station East
Dominique Loqué: Joint BioEnergy Institute, Emery Station East

Nature Communications, 2016, vol. 7, issue 1, 1-8

Abstract: Abstract The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. However, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. Here, we describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.

Date: 2016
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DOI: 10.1038/ncomms13215

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