Evaluating frequency and quality of pathogen-specific T cells

Nadia Anikeeva, Dolores Grosso, Neal Flomenberg () and Yuri Sykulev ()
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Nadia Anikeeva: Thomas Jefferson University
Dolores Grosso: Thomas Jefferson University
Neal Flomenberg: Thomas Jefferson University
Yuri Sykulev: Thomas Jefferson University

Nature Communications, 2016, vol. 7, issue 1, 1-8

Abstract: Abstract It is generally accepted that enumeration and characterization of antigen-specific T cells provide essential information about potency of the immune response. Here, we report a new technique to determine the frequency and potency of antigen-specific CD8 T cells. The assay measures changes of intracellular Ca2+ in real time by fluorescent microscopy in individual CD8 T cells responding to cognate peptides. The T cells form continuous monolayer, enabling the cells to present the peptides to each other. This approach allows us to evaluate the kinetics of intracellular Ca2+ signalling that characterizes the quality of T cell response. We demonstrate the usefulness of the assay examining the frequency and quality of cytomegalovirus-specific CD8 T cells from healthy donor and patient after haploidentical stem cell transplantation. The new assay has a potential to provide essential information determining the status of the immune system, disease morbidity, potency of therapeutic intervention and vaccine efficacy.

Date: 2016
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DOI: 10.1038/ncomms13264

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