DNA looping mediates nucleosome transfer

Lucy D. Brennan, Robert A. Forties, Smita S. Patel and Michelle D. Wang ()
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Lucy D. Brennan: Cornell University
Robert A. Forties: Cornell University
Smita S. Patel: Rutgers-Robert Wood Johnson Medical School
Michelle D. Wang: Cornell University

Nature Communications, 2016, vol. 7, issue 1, 1-8

Abstract: Abstract Proper cell function requires preservation of the spatial organization of chromatin modifications. Maintenance of this epigenetic landscape necessitates the transfer of parental nucleosomes to newly replicated DNA, a process that is stringently regulated and intrinsically linked to replication fork dynamics. This creates a formidable setting from which to isolate the central mechanism of transfer. Here we utilized a minimal experimental system to track the fate of a single nucleosome following its displacement, and examined whether DNA mechanics itself, in the absence of any chaperones or assembly factors, may serve as a platform for the transfer process. We found that the nucleosome is passively transferred to available dsDNA as predicted by a simple physical model of DNA loop formation. These results demonstrate a fundamental role for DNA mechanics in mediating nucleosome transfer and preserving epigenetic integrity during replication.

Date: 2016
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DOI: 10.1038/ncomms13337

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