In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry

Maria Cristina Lo Giudice, Luciana M. Herda, Ester Polo () and Kenneth A. Dawson ()
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Maria Cristina Lo Giudice: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin
Luciana M. Herda: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin
Ester Polo: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin
Kenneth A. Dawson: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented in complex biological milieu, which produce a bio-interface or ‘biomolecular corona’ conferring a biological identity to the particle. Biomolecules at the surface of the nanoparticle–biomolecule complex present molecular fragments that may be recognized by receptors of cells or biological barriers, potentially engaging with different biological pathways. Here we demonstrate that using intense fluorescent reporter binders, in this case antibodies bound to quantum dots, we can map out the availability of such recognition fragments, allowing for a rapid and meaningful biological characterization. The application in microfluidic flow, in small detection volumes, with appropriate thresholding of the detection allows the study of even complex nanoparticles in realistic biological milieu, with the emerging prospect of making direct connection to conditions of cell level and in vivo experiments.

Date: 2016
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DOI: 10.1038/ncomms13475

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