Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction

Marie A. Hanebuth, Roman Kityk, Sandra J. Fries, Alok Jain, Allison Kriel, Veronique Albanese, Tancred Frickey, Christine Peter, Matthias P. Mayer, Judith Frydman and Elke Deuerling ()
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Marie A. Hanebuth: Molecular Microbiology, University of Konstanz
Roman Kityk: Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance
Sandra J. Fries: Molecular Microbiology, University of Konstanz
Alok Jain: University of Konstanz
Allison Kriel: Stanford University
Veronique Albanese: Stanford University
Tancred Frickey: Applied Bioinformatics, University of Konstanz
Christine Peter: University of Konstanz
Matthias P. Mayer: Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance
Judith Frydman: Stanford University
Elke Deuerling: Molecular Microbiology, University of Konstanz

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of Ssb mediates the key contact and a second attachment point is provided by a KRR-motif in the substrate binding domain. Strikingly, ribosome binding of Ssb is not essential. Autonomous ribosome attachment becomes necessary if RAC is absent, suggesting a dual mode of Ssb recruitment to nascent chains. We propose, that the multilayered ribosomal interaction allows positioning of Ssb in an optimal orientation to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.

Date: 2016
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DOI: 10.1038/ncomms13695

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