The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins

Anke Harsman, Silke Oeljeklaus, Christoph Wenger, Jonathan L. Huot, Bettina Warscheid () and André Schneider ()
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Anke Harsman: University of Bern
Silke Oeljeklaus: Institute of Biology II, Faculty of Biology, University of Freiburg
Christoph Wenger: University of Bern
Jonathan L. Huot: University of Bern
Bettina Warscheid: Institute of Biology II, Faculty of Biology, University of Freiburg
André Schneider: University of Bern

Nature Communications, 2016, vol. 7, issue 1, 1-13

Abstract: Abstract Mitochondrial protein import is essential for all eukaryotes. Here we show that the early diverging eukaryote Trypanosoma brucei has a non-canonical inner membrane (IM) protein translocation machinery. Besides TbTim17, the single member of the Tim17/22/23 family in trypanosomes, the presequence translocase contains nine subunits that co-purify in reciprocal immunoprecipitations and with a presequence-containing substrate that is trapped in the translocation channel. Two of the newly discovered subunits are rhomboid-like proteins, which are essential for growth and mitochondrial protein import. Rhomboid-like proteins were proposed to form the protein translocation pore of the ER-associated degradation system, suggesting that they may contribute to pore formation in the presequence translocase of T. brucei. Pulldown of import-arrested mitochondrial carrier protein shows that the carrier translocase shares eight subunits with the presequence translocase. This indicates that T. brucei may have a single IM translocase that with compositional variations mediates import of presequence-containing and carrier proteins.

Date: 2016
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DOI: 10.1038/ncomms13707

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