Five colour variants of bright luminescent protein for real-time multicolour bioimaging

Kazushi Suzuki, Taichi Kimura, Hajime Shinoda, Guirong Bai, Matthew J. Daniels, Yoshiyuki Arai, Masahiro Nakano and Takeharu Nagai ()
Additional contact information
Kazushi Suzuki: Graduate School of Engineering, Osaka University
Taichi Kimura: School of Engineering, Osaka University
Hajime Shinoda: Graduate School of Engineering, Osaka University
Guirong Bai: The Institute of Scientific and Industrial Research, Osaka University
Matthew J. Daniels: BHF Centre for Regenerative Medicine
Yoshiyuki Arai: Graduate School of Engineering, Osaka University
Masahiro Nakano: Graduate School of Engineering, Osaka University
Takeharu Nagai: Graduate School of Engineering, Osaka University

Nature Communications, 2016, vol. 7, issue 1, 1-10

Abstract: Abstract Luminescence imaging has gained attention as a promising bio-imaging modality in situations where fluorescence imaging cannot be applied. However, wider application to multicolour and dynamic imaging is limited by the lack of bright luminescent proteins with emissions across the visible spectrum. Here we report five new spectral variants of the bright luminescent protein, enhanced Nano-lantern (eNL), made by concatenation of the brightest luciferase, NanoLuc, with various colour hues of fluorescent proteins. eNLs allow five-colour live-cell imaging, as well as detection of single protein complexes and even single molecules. We also develop an eNL-based Ca2+ indicator with a 500% signal change, which can image spontaneous Ca2+ dynamics in cardiomyocyte and neural cell models. These eNL probes facilitate not only multicolour imaging in living cells but also sensitive imaging of a wide repertoire of proteins, even at very low expression levels.

Date: 2016
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/ncomms13718 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13718

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms13718

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().