Specialized interfaces of Smc5/6 control hinge stability and DNA association

Aaron Alt, Hung Q. Dang, Owen S. Wells, Luis M. Polo, Matt A. Smith, Grant A. McGregor, Thomas Welte, Alan R. Lehmann, Laurence H. Pearl (), Johanne M. Murray () and Antony W. Oliver ()
Additional contact information
Aaron Alt: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Hung Q. Dang: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Owen S. Wells: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Luis M. Polo: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Matt A. Smith: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Grant A. McGregor: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Thomas Welte: Dynamic Biosensors GmbH, Lochhamer Strasse
Alan R. Lehmann: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Laurence H. Pearl: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Johanne M. Murray: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Antony W. Oliver: Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract The Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6 are involved in the organization of higher-order chromosome structure—which is essential for accurate chromosome duplication and segregation. Each complex is scaffolded by a specific SMC protein dimer (heterodimer in eukaryotes) held together via their hinge domains. Here we show that the Smc5/6-hinge, like those of cohesin and condensin, also forms a toroidal structure but with distinctive subunit interfaces absent from the other SMC complexes; an unusual ‘molecular latch’ and a functional ‘hub’. Defined mutations in these interfaces cause severe phenotypic effects with sensitivity to DNA-damaging agents in fission yeast and reduced viability in human cells. We show that the Smc5/6-hinge complex binds preferentially to ssDNA and that this interaction is affected by both ‘latch’ and ‘hub’ mutations, suggesting a key role for these unique features in controlling DNA association by the Smc5/6 complex.

Date: 2017
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DOI: 10.1038/ncomms14011

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