High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry

Yu-Sang Sabrina Yang, Prabhani U. Atukorale, Kelly D. Moynihan, Ahmet Bekdemir, Kavya Rakhra, Li Tang, Francesco Stellacci and Darrell J. Irvine ()
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Yu-Sang Sabrina Yang: Massachusetts Institute of Technology
Prabhani U. Atukorale: Massachusetts Institute of Technology
Kelly D. Moynihan: Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Ahmet Bekdemir: École Polytechnique Fédérale de Lausanne, Institute of Materials and Interfaculty Bioengineering Institute
Kavya Rakhra: Massachusetts Institute of Technology
Li Tang: Massachusetts Institute of Technology
Francesco Stellacci: École Polytechnique Fédérale de Lausanne, Institute of Materials and Interfaculty Bioengineering Institute
Darrell J. Irvine: Massachusetts Institute of Technology

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Inorganic nanoparticles (NPs) are studied as drug carriers, radiosensitizers and imaging agents, and characterizing nanoparticle biodistribution is essential for evaluating their efficacy and safety. Tracking NPs at the single-cell level with current technologies is complicated by the lack of reliable methods to stably label particles over extended durations in vivo. Here we demonstrate that mass cytometry by time-of-flight provides a label-free approach for inorganic nanoparticle quantitation in cells. Furthermore, mass cytometry can enumerate AuNPs with a lower detection limit of ∼10 AuNPs (3 nm core size) in a single cell with tandem multiparameter cellular phenotyping. Using the cellular distribution insights, we selected an amphiphilic surface ligand-coated AuNP that targeted myeloid dendritic cells in lymph nodes as a peptide antigen carrier, substantially increasing the efficacy of a model vaccine in a B16-OVA melanoma mouse model. This technology provides a powerful new level of insight into nanoparticle fate in vivo.

Date: 2017
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DOI: 10.1038/ncomms14069

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