Whole-brain 3D mapping of human neural transplant innervation

Jonas Doerr, Martin Karl Schwarz, Dirk Wiedermann, Anke Leinhaas, Alina Jakobs, Florian Schloen, Inna Schwarz, Michael Diedenhofen, Nils Christian Braun, Philipp Koch, Daniel A. Peterson, Ulrich Kubitscheck, Mathias Hoehn and Oliver Brüstle ()
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Jonas Doerr: Institute of Reconstructive Neurobiology, University of Bonn
Martin Karl Schwarz: Life&Brain GmbH
Dirk Wiedermann: Max Planck Institute for Metabolism Research, In-vivo-NMR Laboratory
Anke Leinhaas: Institute of Reconstructive Neurobiology, University of Bonn
Alina Jakobs: Institute of Reconstructive Neurobiology, University of Bonn
Florian Schloen: Institute of Physical and Theoretical Chemistry, University of Bonn
Inna Schwarz: Functional Neuroconnectomics Group, University of Bonn
Michael Diedenhofen: Max Planck Institute for Metabolism Research, In-vivo-NMR Laboratory
Nils Christian Braun: Institute of Reconstructive Neurobiology, University of Bonn
Philipp Koch: Institute of Reconstructive Neurobiology, University of Bonn
Daniel A. Peterson: Center for Stem Cell and Regenerative Medicine, Rosalind Franklin University of Medicine and Science
Ulrich Kubitscheck: Institute of Physical and Theoretical Chemistry, University of Bonn
Mathias Hoehn: Max Planck Institute for Metabolism Research, In-vivo-NMR Laboratory
Oliver Brüstle: Institute of Reconstructive Neurobiology, University of Bonn

Nature Communications, 2017, vol. 8, issue 1, 1-7

Abstract: Abstract While transplantation represents a key tool for assessing in vivo functionality of neural stem cells and their suitability for neural repair, little is known about the integration of grafted neurons into the host brain circuitry. Rabies virus-based retrograde tracing has developed into a powerful approach for visualizing synaptically connected neurons. Here, we combine this technique with light sheet fluorescence microscopy (LSFM) to visualize transplanted cells and connected host neurons in whole-mouse brain preparations. Combined with co-registration of high-precision three-dimensional magnetic resonance imaging (3D MRI) reference data sets, this approach enables precise anatomical allocation of the host input neurons. Our data show that the same neural donor cell population grafted into different brain regions receives highly orthotopic input. These findings indicate that transplant connectivity is largely dictated by the circuitry of the target region and depict rabies-based transsynaptic tracing and LSFM as efficient tools for comprehensive assessment of host–donor cell innervation.

Date: 2017
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DOI: 10.1038/ncomms14162

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