Dynamic behaviour of human neuroepithelial cells in the developing forebrain

Lakshmi Subramanian (), Marina Bershteyn, Mercedes F. Paredes and Arnold R. Kriegstein ()
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Lakshmi Subramanian: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
Marina Bershteyn: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
Mercedes F. Paredes: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco
Arnold R. Kriegstein: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract To understand how diverse progenitor cells contribute to human neocortex development, we examined forebrain progenitor behaviour using timelapse imaging. Here we find that cell cycle dynamics of human neuroepithelial (NE) cells differ from radial glial (RG) cells in both primary tissue and in stem cell-derived organoids. NE cells undergoing proliferative, symmetric divisions retract their basal processes, and both daughter cells regrow a new process following cytokinesis. The mitotic retraction of the basal process is recapitulated by NE cells in cerebral organoids generated from human-induced pluripotent stem cells. In contrast, RG cells undergoing vertical cleavage retain their basal fibres throughout mitosis, both in primary tissue and in older organoids. Our findings highlight developmentally regulated changes in mitotic behaviour that may relate to the role of RG cells to provide a stable scaffold for neuronal migration, and suggest that the transition in mitotic dynamics can be studied in organoid models.

Date: 2017
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DOI: 10.1038/ncomms14167

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