A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

Chen Li, Yusuke Sako, Akihiro Imai, Tomoaki Nishiyama, Kari Thompson, Minoru Kubo, Yuji Hiwatashi, Yukiko Kabeya, Dale Karlson, Shu-Hsing Wu, Masaki Ishikawa, Takashi Murata, Philip N. Benfey, Yoshikatsu Sato, Yosuke Tamada () and Mitsuyasu Hasebe ()
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Chen Li: National Institute for Basic Biology
Yusuke Sako: National Institute for Basic Biology
Akihiro Imai: National Institute for Basic Biology
Tomoaki Nishiyama: ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency
Kari Thompson: National Institute for Basic Biology
Minoru Kubo: National Institute for Basic Biology
Yuji Hiwatashi: National Institute for Basic Biology
Yukiko Kabeya: National Institute for Basic Biology
Dale Karlson: West Virginia University
Shu-Hsing Wu: Institute of Plant and Microbial Biology, Academia Sinica
Masaki Ishikawa: National Institute for Basic Biology
Takashi Murata: National Institute for Basic Biology
Philip N. Benfey: Duke University
Yoshikatsu Sato: National Institute for Basic Biology
Yosuke Tamada: National Institute for Basic Biology
Mitsuyasu Hasebe: National Institute for Basic Biology

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

Date: 2017
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DOI: 10.1038/ncomms14242

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