The tumour suppressor APC promotes HIV-1 assembly via interaction with Gag precursor protein

Kei Miyakawa, Mayuko Nishi, Satoko Matsunaga, Akiko Okayama, Masaki Anraku, Ayumi Kudoh, Hisashi Hirano, Hirokazu Kimura, Yuko Morikawa, Naoki Yamamoto, Akira Ono and Akihide Ryo ()
Additional contact information
Kei Miyakawa: Yokohama City University School of Medicine
Mayuko Nishi: Yokohama City University School of Medicine
Satoko Matsunaga: Yokohama City University School of Medicine
Akiko Okayama: Advanced Medical Research Center, Yokohama City University
Masaki Anraku: Yokohama City University School of Medicine
Ayumi Kudoh: Yokohama City University School of Medicine
Hisashi Hirano: Advanced Medical Research Center, Yokohama City University
Hirokazu Kimura: Infectious Disease Surveillance Center, National Institute of Infectious Diseases
Yuko Morikawa: Kitasato Institute for Life Sciences and Graduate School for Infection Control, Kitasato University
Naoki Yamamoto: National University of Singapore
Akira Ono: University of Michigan Medical School
Akihide Ryo: Yokohama City University School of Medicine

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract Diverse cellular proteins and RNAs are tightly regulated in their subcellular localization to exert their local function. Here we report that the tumour suppressor adenomatous polyposis coli protein (APC) directs the localization and assembly of human immunodeficiency virus (HIV)-1 Gag polyprotein at distinct membrane components to enable the efficient production and spread of infectious viral particles. A proteomic analysis and subsequent biomolecular interaction assay reveals that the carboxyl terminus of APC interacts with the matrix region of Gag. Ectopic expression of APC, but not its familial adenomatous polyposis-related truncation mutant, prominently enhances HIV-1 production. Conversely, the depletion of APC leads to a significant decrease in membrane targeting of viral components, resulting in the severe loss of production of infectious virions. Furthermore, APC promotes the directional assembly of viral components at virological synapses, thereby facilitating cell-to-cell viral transmission. These findings reveal an unexpected role of APC in the directional spread of HIV-1.

Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms14259 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14259

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms14259

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().