Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions

Andreas Sprengel, Pascal Lill, Pierre Stegemann, Kenny Bravo-Rodriguez, Elisa-C. Schöneweiß, Melisa Merdanovic, Daniel Gudnason, Mikayel Aznauryan, Lisa Gamrad, Stephan Barcikowski, Elsa Sanchez-Garcia, Victoria Birkedal, Christos Gatsogiannis, Michael Ehrmann and Barbara Saccà ()
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Andreas Sprengel: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Pascal Lill: Max-Planck-Institute of Molecular Physiology
Pierre Stegemann: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Kenny Bravo-Rodriguez: Max-Planck-Institut für Kohlenforschung
Elisa-C. Schöneweiß: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Melisa Merdanovic: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Daniel Gudnason: Aarhus University
Mikayel Aznauryan: Aarhus University
Lisa Gamrad: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Stephan Barcikowski: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Elsa Sanchez-Garcia: Max-Planck-Institut für Kohlenforschung
Victoria Birkedal: Aarhus University
Christos Gatsogiannis: Max-Planck-Institute of Molecular Physiology
Michael Ehrmann: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen
Barbara Saccà: Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. However, current strategies of DNA–protein conjugation still limit true emulation of natural host–guest systems, whose formation relies on non-covalent bonds between geometrically matching interfaces. Here we report one of the largest DNA–protein complexes of semisynthetic origin held in place exclusively by spatially defined supramolecular interactions. Our approach is based on the decoration of the inner surface of a DNA origami hollow structure with multiple ligands converging to their corresponding binding sites on the protein surface with programmable symmetry and range-of-action. Our results demonstrate specific host–guest recognition in a 1:1 stoichiometry and selectivity for the guest whose size guarantees sufficient molecular diffusion preserving short intermolecular distances. DNA nanocontainers can be thus rationally designed to trap single guest molecules in their native form, mimicking natural strategies of molecular recognition and anticipating a new method of protein caging.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14472

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DOI: 10.1038/ncomms14472

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