Expanding the genetic code of Mus musculus

Songmi Han, Aerin Yang, Soonjang Lee, Han-Woong Lee, Chan Bae Park () and Hee-Sung Park ()
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Songmi Han: Ajou University School of Medicine
Aerin Yang: Korea Advanced Institute of Science and Technology
Soonjang Lee: Ajou University School of Medicine
Han-Woong Lee: Yonsei Laboratory Animal Research Center, Yonsei University
Chan Bae Park: Ajou University School of Medicine
Hee-Sung Park: Korea Advanced Institute of Science and Technology

Nature Communications, 2017, vol. 8, issue 1, 1-7

Abstract: Abstract Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Nɛ-acetyl-lysine. Stable integration of transgenes encoding an engineered Nɛ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.

Date: 2017
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DOI: 10.1038/ncomms14568

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