A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

Shuo Xiao, Jonathan R. Coppeta, Hunter B. Rogers, Brett C. Isenberg, Jie Zhu, Susan A. Olalekan, Kelly E. McKinnon, Danijela Dokic, Alexandra S. Rashedi, Daniel J. Haisenleder, Saurabh S. Malpani, Chanel A. Arnold-Murray, Kuanwei Chen, Mingyang Jiang, Lu Bai, Catherine T. Nguyen, Jiyang Zhang, Monica M. Laronda, Thomas J. Hope, Kruti P. Maniar, Mary Ellen Pavone, Michael J. Avram, Elizabeth C. Sefton, Spiro Getsios, Joanna E. Burdette, J. Julie Kim, Jeffrey T. Borenstein and Teresa K. Woodruff ()
Additional contact information
Shuo Xiao: Feinberg School of Medicine, Northwestern University
Jonathan R. Coppeta: The Charles Stark Draper Laboratory
Hunter B. Rogers: Feinberg School of Medicine, Northwestern University
Brett C. Isenberg: The Charles Stark Draper Laboratory
Jie Zhu: Feinberg School of Medicine, Northwestern University
Susan A. Olalekan: Feinberg School of Medicine, Northwestern University
Kelly E. McKinnon: Feinberg School of Medicine, Northwestern University
Danijela Dokic: Feinberg School of Medicine, Northwestern University
Alexandra S. Rashedi: Feinberg School of Medicine, Northwestern University
Daniel J. Haisenleder: Ligand Assay and Analysis Core, Center for Research in Reproduction, University of Virginia
Saurabh S. Malpani: Feinberg School of Medicine, Northwestern University
Chanel A. Arnold-Murray: Feinberg School of Medicine, Northwestern University
Kuanwei Chen: Feinberg School of Medicine, Northwestern University
Mingyang Jiang: Feinberg School of Medicine, Northwestern University
Lu Bai: Feinberg School of Medicine, Northwestern University
Catherine T. Nguyen: Feinberg School of Medicine, Northwestern University
Jiyang Zhang: Feinberg School of Medicine, Northwestern University
Monica M. Laronda: Feinberg School of Medicine, Northwestern University
Thomas J. Hope: Feinberg School of Medicine, Northwestern University
Kruti P. Maniar: Feinberg School of Medicine, Northwestern University
Mary Ellen Pavone: Feinberg School of Medicine, Northwestern University
Michael J. Avram: Feinberg School of Medicine, Northwestern University
Elizabeth C. Sefton: Feinberg School of Medicine, Northwestern University
Spiro Getsios: Feinberg School of Medicine, Northwestern University
Joanna E. Burdette: University of Illinois at Chicago
J. Julie Kim: Feinberg School of Medicine, Northwestern University
Jeffrey T. Borenstein: The Charles Stark Draper Laboratory
Teresa K. Woodruff: Feinberg School of Medicine, Northwestern University

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract The endocrine system dynamically controls tissue differentiation and homeostasis, but has not been studied using dynamic tissue culture paradigms. Here we show that a microfluidic system supports murine ovarian follicles to produce the human 28-day menstrual cycle hormone profile, which controls human female reproductive tract and peripheral tissue dynamics in single, dual and multiple unit microfluidic platforms (Solo-MFP, Duet-MFP and Quintet-MPF, respectively). These systems simulate the in vivo female reproductive tract and the endocrine loops between organ modules for the ovary, fallopian tube, uterus, cervix and liver, with a sustained circulating flow between all tissues. The reproductive tract tissues and peripheral organs integrated into a microfluidic platform, termed EVATAR, represents a powerful new in vitro tool that allows organ–organ integration of hormonal signalling as a phenocopy of menstrual cycle and pregnancy-like endocrine loops and has great potential to be used in drug discovery and toxicology studies.

Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms14584 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14584

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms14584

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().