Laser-mediated rupture of chlamydial inclusions triggers pathogen egress and host cell necrosis

Markus C. Kerr, Guillermo A. Gomez, Charles Ferguson, Maria C. Tanzer, James M. Murphy, Alpha S. Yap, Robert G. Parton, Wilhelmina M. Huston and Rohan D Teasdale ()
Additional contact information
Markus C. Kerr: Institute for Molecular Bioscience, The University of Queensland
Guillermo A. Gomez: Institute for Molecular Bioscience, The University of Queensland
Charles Ferguson: Institute for Molecular Bioscience, The University of Queensland
Maria C. Tanzer: The Walter and Eliza Hall Institute of Medical Research
James M. Murphy: The Walter and Eliza Hall Institute of Medical Research
Alpha S. Yap: Institute for Molecular Bioscience, The University of Queensland
Robert G. Parton: Institute for Molecular Bioscience, The University of Queensland
Wilhelmina M. Huston: School of Life Sciences, University of Technology Sydney
Rohan D Teasdale: Institute for Molecular Bioscience, The University of Queensland

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Remarkably little is known about how intracellular pathogens exit the host cell in order to infect new hosts. Pathogenic chlamydiae egress by first rupturing their replicative niche (the inclusion) before rapidly lysing the host cell. Here we apply a laser ablation strategy to specifically disrupt the chlamydial inclusion, thereby uncoupling inclusion rupture from the subsequent cell lysis and allowing us to dissect the molecular events involved in each step. Pharmacological inhibition of host cell calpains inhibits inclusion rupture, but not subsequent cell lysis. Further, we demonstrate that inclusion rupture triggers a rapid necrotic cell death pathway independent of BAK, BAX, RIP1 and caspases. Both processes work sequentially to efficiently liberate the pathogen from the host cytoplasm, promoting secondary infection. These results reconcile the pathogen's known capacity to promote host cell survival and induce cell death.

Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms14729 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14729

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms14729

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().