An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

Kongtana Trakarnsanga, Rebecca E. Griffiths, Marieangela C. Wilson, Allison Blair, Timothy J. Satchwell, Marjolein Meinders, Nicola Cogan, Sabine Kupzig, Ryo Kurita, Yukio Nakamura, Ashley M. Toye, David J. Anstee () and Jan Frayne ()
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Kongtana Trakarnsanga: School of Biochemistry, University of Bristol
Rebecca E. Griffiths: Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT)
Marieangela C. Wilson: School of Biochemistry, University of Bristol
Allison Blair: Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT)
Timothy J. Satchwell: School of Biochemistry, University of Bristol
Marjolein Meinders: School of Biochemistry, University of Bristol
Nicola Cogan: Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT)
Sabine Kupzig: Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT)
Ryo Kurita: Central Blood Institute, Japanese Red Cross Society
Yukio Nakamura: RIKEN BioResource Center
Ashley M. Toye: School of Biochemistry, University of Bristol
David J. Anstee: Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT)
Jan Frayne: School of Biochemistry, University of Bristol

Nature Communications, 2017, vol. 8, issue 1, 1-7

Abstract: Abstract With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.

Date: 2017
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DOI: 10.1038/ncomms14750

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