Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome

Florian Douam, Gabriela Hrebikova, Yentli E. Soto Albrecht, Julie Sellau, Yael Sharon, Qiang Ding and Alexander Ploss ()
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Florian Douam: Princeton University, 110 Lewis Thomas Laboratory
Gabriela Hrebikova: Princeton University, 110 Lewis Thomas Laboratory
Yentli E. Soto Albrecht: Princeton University, 110 Lewis Thomas Laboratory
Julie Sellau: Princeton University, 110 Lewis Thomas Laboratory
Yael Sharon: Princeton University, 110 Lewis Thomas Laboratory
Qiang Ding: Princeton University, 110 Lewis Thomas Laboratory
Alexander Ploss: Princeton University, 110 Lewis Thomas Laboratory

Nature Communications, 2017, vol. 8, issue 1, 1-17

Abstract: Abstract Positive-sense RNA viruses pose increasing health and economic concerns worldwide. Our limited understanding of how these viruses interact with their host and how these processes lead to virulence and disease seriously hampers the development of anti-viral strategies. Here, we demonstrate the tracking of (+) and (−) sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models. Our results provide insights into how a prototypic flavivirus, yellow fever virus (YFV-17D), differentially interacts with murine and human hematopoietic cells in these mouse models and how these dynamics influence distinct outcomes of infection. We detect (−) YFV-17D RNA in specific secondary lymphoid compartments and cell subsets not previously recognized as permissive for YFV replication, and we highlight potential virus–host interaction events that could be pivotal in regulating flavivirus virulence and attenuation.

Date: 2017
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DOI: 10.1038/ncomms14781

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