Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

P. Guichard (), V. Hamel, M. Le Guennec, N. Banterle, I. Iacovache, V. Nemčíková, I. Flückiger, K. N. Goldie, H. Stahlberg, D. Lévy, B. Zuber and P. Gönczy ()
Additional contact information
P. Guichard: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
V. Hamel: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
M. Le Guennec: Sciences III, University of Geneva
N. Banterle: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
I. Iacovache: Institute of Anatomy, University of Bern
V. Nemčíková: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
I. Flückiger: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
K. N. Goldie: Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel
H. Stahlberg: Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel
D. Lévy: Institut Curie, PSL Research University, UMR 168, Centre de Recherche, 26 rue d’ULM, Paris 75231, France
B. Zuber: Institute of Anatomy, University of Bern
P. Gönczy: Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-fold symmetrical cartwheel structure typically ∼100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 proteins. Whether nine-fold symmetrical ring-like assemblies of SAS-6 proteins harbour more peripheral cartwheel elements is unclear. Furthermore, the mechanisms governing ring stacking are not known. Here we develop a cell-free reconstitution system for core cartwheel assembly. Using cryo-electron tomography, we uncover that the Chlamydomonas reinhardtii proteins CrSAS-6 and Bld10p together drive assembly of the core cartwheel. Moreover, we discover that CrSAS-6 possesses autonomous properties that ensure self-organized ring stacking. Mathematical fitting of reconstituted cartwheel height distribution suggests a mechanism whereby preferential addition of pairs of SAS-6 rings governs cartwheel growth. In conclusion, we have developed a cell-free reconstitution system that reveals fundamental assembly principles at the root of centriole biogenesis.

Date: 2017
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DOI: 10.1038/ncomms14813

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