A Pseudomonas T6SS effector recruits PQS-containing outer membrane vesicles for iron acquisition

Jinshui Lin, Weipeng Zhang, Juanli Cheng, Xu Yang, Kaixiang Zhu, Yao Wang, Gehong Wei, Pei-Yuan Qian, Zhao-Qing Luo and Xihui Shen ()
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Jinshui Lin: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Weipeng Zhang: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Juanli Cheng: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Xu Yang: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Kaixiang Zhu: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Yao Wang: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Gehong Wei: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University
Pei-Yuan Qian: Hong Kong University of Science and Technology
Zhao-Qing Luo: Purdue Institute of Immunology, Purdue University
Xihui Shen: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Iron sequestration by host proteins contributes to the defence against bacterial pathogens, which need iron for their metabolism and virulence. A Pseudomonas aeruginosa mutant lacking all three known iron acquisition systems retains the ability to grow in media containing iron chelators, suggesting the presence of additional pathways involved in iron uptake. Here we screen P. aeruginosa mutants defective in growth in iron-depleted media and find that gene PA2374, proximal to the type VI secretion system H3 (H3-T6SS), functions synergistically with known iron acquisition systems. PA2374 (which we have renamed TseF) appears to be secreted by H3-T6SS and is incorporated into outer membrane vesicles (OMVs) by directly interacting with the iron-binding Pseudomonas quinolone signal (PQS), a cell–cell signalling compound. TseF facilitates the delivery of OMV-associated iron to bacterial cells by engaging the Fe(III)-pyochelin receptor FptA and the porin OprF. Our results reveal links between type VI secretion, cell–cell signalling and classic siderophore receptors for iron acquisition in P. aeruginosa.

Date: 2017
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DOI: 10.1038/ncomms14888

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