Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models

In Young Hwang, Elvin Koh, Adison Wong, John C. March, William E. Bentley, Yung Seng Lee and Matthew Wook Chang ()
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In Young Hwang: Yong Loo Lin School of Medicine, National University of Singapore
Elvin Koh: Yong Loo Lin School of Medicine, National University of Singapore
Adison Wong: Yong Loo Lin School of Medicine, National University of Singapore
John C. March: Cornell University
William E. Bentley: University of Maryland
Yung Seng Lee: NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore
Matthew Wook Chang: Yong Loo Lin School of Medicine, National University of Singapore

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice). These findings support the further development of engineered microorganisms with potential prophylactic and therapeutic activities against gut infections.

Date: 2017
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DOI: 10.1038/ncomms15028

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