Joint morphogenetic cells in the adult mammalian synovium

Anke J. Roelofs, Janja Zupan, Anna H. K. Riemen, Karolina Kania, Sharon Ansboro, Nathan White, Susan M. Clark and Cosimo De Bari ()
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Anke J. Roelofs: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Janja Zupan: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Anna H. K. Riemen: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Karolina Kania: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Sharon Ansboro: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Nathan White: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Susan M. Clark: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen
Cosimo De Bari: Arthritis & Regenerative Medicine Laboratory, Institute of Medical Sciences, University of Aberdeen

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract The stem cells that safeguard synovial joints in adulthood are undefined. Studies on mesenchymal stromal/stem cells (MSCs) have mainly focused on bone marrow. Here we show that lineage tracing of Gdf5-expressing joint interzone cells identifies in adult mouse synovium an MSC population largely negative for the skeletal stem cell markers Nestin-GFP, Leptin receptor and Gremlin1. Following cartilage injury, Gdf5-lineage cells underpin synovial hyperplasia through proliferation, are recruited to a Nestin-GFPhigh perivascular population, and contribute to cartilage repair. The transcriptional co-factor Yap is upregulated after injury, and its conditional ablation in Gdf5-lineage cells prevents synovial lining hyperplasia and decreases contribution of Gdf5-lineage cells to cartilage repair. Cultured Gdf5-lineage cells exhibit progenitor activity for stable chondrocytes and are able to self-organize three-dimensionally to form a synovial lining-like layer. Finally, human synovial MSCs transduced with Bmp7 display morphogenetic properties by patterning a joint-like organ in vivo. Our findings further the understanding of the skeletal stem/progenitor cells in adult life.

Date: 2017
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DOI: 10.1038/ncomms15040

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