Distinct molecular cues ensure a robust microtubule-dependent nuclear positioning in the Drosophila oocyte

Nicolas Tissot, Jean-Antoine Lepesant, Fred Bernard, Kevin Legent, Floris Bosveld, Charlotte Martin, Orestis Faklaris, Yohanns Bellaïche, Maïté Coppey and Antoine Guichet ()
Additional contact information
Nicolas Tissot: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité
Jean-Antoine Lepesant: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité
Fred Bernard: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité
Kevin Legent: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité
Floris Bosveld: Polarity, Institut Curie, CNRS UMR 3215, INSERM U934
Charlotte Martin: Polarity, Institut Curie, CNRS UMR 3215, INSERM U934
Orestis Faklaris: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité
Yohanns Bellaïche: Polarity, Institut Curie, CNRS UMR 3215, INSERM U934
Maïté Coppey: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité
Antoine Guichet: Polarity and Morphogenesis Team, Institut Jacques Monod, CNRS UMR 7592, Université Paris-Diderot, Sorbonne sParis Cité

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract Controlling nucleus localization is crucial for a variety of cellular functions. In the Drosophila oocyte, nuclear asymmetric positioning is essential for the reorganization of the microtubule (MT) network that controls the polarized transport of axis determinants. A combination of quantitative three-dimensional live imaging and laser ablation-mediated force analysis reveal that nuclear positioning is ensured with an unexpected level of robustness. We show that the nucleus is pushed to the oocyte antero-dorsal cortex by MTs and that its migration can proceed through distinct tracks. Centrosome-associated MTs favour one migratory route. In addition, the MT-associated protein Mud/NuMA that is asymmetrically localized in an Asp-dependent manner at the nuclear envelope hemisphere where MT nucleation is higher promotes a separate route. Our results demonstrate that centrosomes do not provide an obligatory driving force for nuclear movement, but together with Mud, contribute to the mechanisms that ensure the robustness of asymmetric nuclear positioning.

Date: 2017
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms15168 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15168

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms15168

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().