Maternal age-dependent APC/C-mediated decrease in securin causes premature sister chromatid separation in meiosis II

Ibtissem Nabti (), Rosanna Grimes, Hema Sarna, Petros Marangos and John Carroll ()
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Ibtissem Nabti: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University
Rosanna Grimes: University College London
Hema Sarna: University College London
Petros Marangos: University College London
John Carroll: Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract Sister chromatid attachment during meiosis II (MII) is maintained by securin-mediated inhibition of separase. In maternal ageing, oocytes show increased inter-sister kinetochore distance and premature sister chromatid separation (PSCS), suggesting aberrant separase activity. Here, we find that MII oocytes from aged mice have less securin than oocytes from young mice and that this reduction is mediated by increased destruction by the anaphase promoting complex/cyclosome (APC/C) during meiosis I (MI) exit. Inhibition of the spindle assembly checkpoint (SAC) kinase, Mps1, during MI exit in young oocytes replicates this phenotype. Further, over-expression of securin or Mps1 protects against the age-related increase in inter-sister kinetochore distance and PSCS. These findings show that maternal ageing compromises the oocyte SAC–APC/C axis leading to a decrease in securin that ultimately causes sister chromatid cohesion loss. Manipulating this axis and/or increasing securin may provide novel therapeutic approaches to alleviating the risk of oocyte aneuploidy in maternal ageing.

Date: 2017
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DOI: 10.1038/ncomms15346

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