Time-programmable drug dosing allows the manipulation, suppression and reversal of antibiotic drug resistance in vitro

Mari Yoshida, Sabrina Galiñanes Reyes, Soichiro Tsuda (), Takaaki Horinouchi, Chikara Furusawa and Leroy Cronin ()
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Mari Yoshida: WestCHEM, School of Chemistry, The University of Glasgow
Sabrina Galiñanes Reyes: WestCHEM, School of Chemistry, The University of Glasgow
Soichiro Tsuda: WestCHEM, School of Chemistry, The University of Glasgow
Takaaki Horinouchi: Quantitative Biology Center, RIKEN
Chikara Furusawa: Quantitative Biology Center, RIKEN
Leroy Cronin: WestCHEM, School of Chemistry, The University of Glasgow

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Multi-drug strategies have been attempted to prolong the efficacy of existing antibiotics, but with limited success. Here we show that the evolution of multi-drug-resistant Escherichia coli can be manipulated in vitro by administering pairs of antibiotics and switching between them in ON/OFF manner. Using a multiplexed cell culture system, we find that switching between certain combinations of antibiotics completely suppresses the development of resistance to one of the antibiotics. Using this data, we develop a simple deterministic model, which allows us to predict the fate of multi-drug evolution in this system. Furthermore, we are able to reverse established drug resistance based on the model prediction by modulating antibiotic selection stresses. Our results support the idea that the development of antibiotic resistance may be potentially controlled via continuous switching of drugs.

Date: 2017
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DOI: 10.1038/ncomms15589

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