GroEL actively stimulates folding of the endogenous substrate protein PepQ

Jeremy Weaver, Mengqiu Jiang, Andrew Roth, Jason Puchalla, Junjie Zhang () and Hays S. Rye ()
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Jeremy Weaver: Texas A&M University
Mengqiu Jiang: Texas A&M University
Andrew Roth: Texas A&M University
Jason Puchalla: Princeton University
Junjie Zhang: Texas A&M University
Hays S. Rye: Texas A&M University

Nature Communications, 2017, vol. 8, issue 1, 1-15

Abstract: Abstract Many essential proteins cannot fold without help from chaperonins, like the GroELS system of Escherichia coli. How chaperonins accelerate protein folding remains controversial. Here we test key predictions of both passive and active models of GroELS-stimulated folding, using the endogenous E. coli metalloprotease PepQ. While GroELS increases the folding rate of PepQ by over 15-fold, we demonstrate that slow spontaneous folding of PepQ is not caused by aggregation. Fluorescence measurements suggest that, when folding inside the GroEL-GroES cavity, PepQ populates conformations not observed during spontaneous folding in free solution. Using cryo-electron microscopy, we show that the GroEL C-termini make physical contact with the PepQ folding intermediate and help retain it deep within the GroEL cavity, resulting in reduced compactness of the PepQ monomer. Our findings strongly support an active model of chaperonin-mediated protein folding, where partial unfolding of misfolded intermediates plays a key role.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15934

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DOI: 10.1038/ncomms15934

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