Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

Stefanie L. Morgan, Natasha C. Mariano, Abel Bermudez, Nicole L. Arruda, Fangting Wu, Yunhai Luo, Gautam Shankar, Lin Jia, Huiling Chen, Ji-Fan Hu, Andrew R. Hoffman, Chiao-Chain Huang, Sharon J. Pitteri and Kevin C. Wang ()
Additional contact information
Stefanie L. Morgan: Program in Epithelial Biology, Stanford University School of Medicine
Natasha C. Mariano: Program in Epithelial Biology, Stanford University School of Medicine
Abel Bermudez: Canary Center for Cancer Early Detection, Stanford University School of Medicine
Nicole L. Arruda: Bridgewater State University
Fangting Wu: System Biosciences
Yunhai Luo: Program in Epithelial Biology, Stanford University School of Medicine
Gautam Shankar: Program in Epithelial Biology, Stanford University School of Medicine
Lin Jia: Veterans Affairs Healthcare System
Huiling Chen: Veterans Affairs Healthcare System
Ji-Fan Hu: Veterans Affairs Healthcare System
Andrew R. Hoffman: Veterans Affairs Healthcare System
Chiao-Chain Huang: System Biosciences
Sharon J. Pitteri: Canary Center for Cancer Early Detection, Stanford University School of Medicine
Kevin C. Wang: Program in Epithelial Biology, Stanford University School of Medicine

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

Date: 2017
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DOI: 10.1038/ncomms15993

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