DNA binding drives the association of BRG1/hBRM bromodomains with nucleosomes

Emma A. Morrison, Julio C. Sanchez, Jehnna L. Ronan, Daniel P. Farrell, Katayoun Varzavand, Jenna K. Johnson, Brian X. Gu, Gerald R. Crabtree and Catherine A. Musselman ()
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Emma A. Morrison: Carver College of Medicine, University of Iowa
Julio C. Sanchez: Carver College of Medicine, University of Iowa
Jehnna L. Ronan: Program in Cancer Biology, Stanford University School of Medicine
Daniel P. Farrell: Carver College of Medicine, University of Iowa
Katayoun Varzavand: Carver College of Medicine, University of Iowa
Jenna K. Johnson: Carver College of Medicine, University of Iowa
Brian X. Gu: Carver College of Medicine, University of Iowa
Gerald R. Crabtree: Program in Cancer Biology, Stanford University School of Medicine
Catherine A. Musselman: Carver College of Medicine, University of Iowa

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract BRG1 and BRM, central components of the BAF (mSWI/SNF) chromatin remodelling complex, are critical in chromatin structure regulation. Here, we show that the human BRM (hBRM) bromodomain (BRD) has moderate specificity for H3K14ac. Surprisingly, we also find that both BRG1 and hBRM BRDs have DNA-binding activity. We demonstrate that the BRDs associate with DNA through a surface basic patch and that the BRD and an adjacent AT-hook make multivalent contacts with DNA, leading to robust affinity and moderate specificity for AT-rich elements. Although we show that the BRDs can bind to both DNA and H3K14ac simultaneously, the histone-binding activity does not contribute substantially to nucleosome targeting in vitro. In addition, we find that neither BRD histone nor DNA binding contribute to the global chromatin affinity of BRG1 in mouse embryonic stem cells. Together, our results suggest that association of the BRG1/hBRM BRD with nucleosomes plays a regulatory rather than targeting role in BAF activity.

Date: 2017
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DOI: 10.1038/ncomms16080

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