Quantitative real-time imaging of glutathione

Xiqian Jiang, Jianwei Chen, Aleksandar Bajić, Chengwei Zhang, Xianzhou Song, Shaina L. Carroll, Zhao-Lin Cai, Meiling Tang, Mingshan Xue, Ninghui Cheng, Christian P. Schaaf, Feng Li, Kevin R. MacKenzie, Allan Chris M. Ferreon, Fan Xia, Meng C. Wang, Mirjana Maletić-Savatić and Jin Wang ()
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Xiqian Jiang: Baylor College of Medicine
Jianwei Chen: Baylor College of Medicine
Aleksandar Bajić: Baylor College of Medicine
Chengwei Zhang: Baylor College of Medicine
Xianzhou Song: Baylor College of Medicine
Shaina L. Carroll: Baylor College of Medicine
Zhao-Lin Cai: Baylor College of Medicine
Meiling Tang: Baylor College of Medicine
Mingshan Xue: Baylor College of Medicine
Ninghui Cheng: Baylor College of Medicine
Christian P. Schaaf: Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital
Feng Li: Baylor College of Medicine
Kevin R. MacKenzie: Center for Drug Discovery, Baylor College of Medicine
Allan Chris M. Ferreon: Baylor College of Medicine
Fan Xia: Baylor College of Medicine
Meng C. Wang: Baylor College of Medicine
Mirjana Maletić-Savatić: Baylor College of Medicine
Jin Wang: Baylor College of Medicine

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe—designated as RealThiol (RT)—that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

Date: 2017
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DOI: 10.1038/ncomms16087

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