Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine

Johanna Marsian, Helen Fox, Mohammad W. Bahar, Abhay Kotecha, Elizabeth E. Fry, David I. Stuart, Andrew J. Macadam, David J. Rowlands and George P. Lomonossoff ()
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Johanna Marsian: Norwich Research Park
Helen Fox: The National Institute for Biological Standards and Control
Mohammad W. Bahar: University of Oxford, The Henry Wellcome Building for Genomic Medicine
Abhay Kotecha: University of Oxford, The Henry Wellcome Building for Genomic Medicine
Elizabeth E. Fry: University of Oxford, The Henry Wellcome Building for Genomic Medicine
David I. Stuart: University of Oxford, The Henry Wellcome Building for Genomic Medicine
Andrew J. Macadam: The National Institute for Biological Standards and Control
David J. Rowlands: University of Leeds
George P. Lomonossoff: Norwich Research Park

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced stabilized virus-like particle (sVLP)-based vaccine with D antigenicity, without the drawbacks of current vaccines, will be a major step towards the final eradication of poliovirus. Such a sVLP would retain the native antigenic conformation and the repetitive structure of the original virus particle, but lack infectious genomic material. In this study, we report the production of synthetically stabilized PV VLPs in plants. Mice carrying the gene for the human PV receptor are protected from wild-type PV when immunized with the plant-made PV sVLPs. Structural analysis of the stabilized mutant at 3.6 Å resolution by cryo-electron microscopy and single-particle reconstruction reveals a structure almost indistinguishable from wild-type PV3.

Date: 2017
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DOI: 10.1038/s41467-017-00090-w

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