Negri bodies are viral factories with properties of liquid organelles

Jovan Nikolic, Romain Le Bars, Zoé Lama, Nathalie Scrima, Cécile Lagaudrière-Gesbert, Yves Gaudin () and Danielle Blondel ()
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Jovan Nikolic: Univ. Paris-Sud, Université Paris-Saclay
Romain Le Bars: Univ. Paris-Sud, Université Paris-Saclay
Zoé Lama: Univ. Paris-Sud, Université Paris-Saclay
Nathalie Scrima: Univ. Paris-Sud, Université Paris-Saclay
Cécile Lagaudrière-Gesbert: Univ. Paris-Sud, Université Paris-Saclay
Yves Gaudin: Univ. Paris-Sud, Université Paris-Saclay
Danielle Blondel: Univ. Paris-Sud, Université Paris-Saclay

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract Replication of Mononegavirales occurs in viral factories which form inclusions in the host-cell cytoplasm. For rabies virus, those inclusions are called Negri bodies (NBs). We report that NBs have characteristics similar to those of liquid organelles: they are spherical, they fuse to form larger structures, and they disappear upon hypotonic shock. Their liquid phase is confirmed by FRAP experiments. Live-cell imaging indicates that viral nucleocapsids are ejected from NBs and transported along microtubules to form either new virions or secondary viral factories. Coexpression of rabies virus N and P proteins results in cytoplasmic inclusions recapitulating NBs properties. This minimal system reveals that an intrinsically disordered domain and the dimerization domain of P are essential for Negri bodies-like structures formation. We suggest that formation of liquid viral factories by phase separation is common among Mononegavirales and allows specific recruitment and concentration of viral proteins but also the escape to cellular antiviral response.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00102-9

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DOI: 10.1038/s41467-017-00102-9

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