Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system

Yihan Zhang, Wei Qin, Xiaochan Lu, Jason Xu, Haigen Huang, Haipeng Bai, Song Li and Shuo Lin ()
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Yihan Zhang: Peking University Shenzhen Graduate School
Wei Qin: Peking University Shenzhen Graduate School
Xiaochan Lu: Peking University Shenzhen Graduate School
Jason Xu: University of California
Haigen Huang: University of California
Haipeng Bai: Peking University Shenzhen Graduate School
Song Li: Peking University Shenzhen Graduate School
Shuo Lin: Peking University Shenzhen Graduate School

Nature Communications, 2017, vol. 8, issue 1, 1-5

Abstract: Abstract Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable “base editing” system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5′-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.

Date: 2017
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DOI: 10.1038/s41467-017-00175-6

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