A pluripotent stem cell-based model for post-implantation human amniotic sac development

Yue Shao, Kenichiro Taniguchi, Ryan F. Townshend, Toshio Miki, Deborah L. Gumucio () and Jianping Fu ()
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Yue Shao: University of Michigan
Kenichiro Taniguchi: University of Michigan Medical School
Ryan F. Townshend: University of Michigan Medical School
Toshio Miki: University of Southern California
Deborah L. Gumucio: University of Michigan Medical School
Jianping Fu: University of Michigan

Nature Communications, 2017, vol. 8, issue 1, 1-15

Abstract: Abstract Development of the asymmetric amniotic sac—with the embryonic disc and amniotic ectoderm occupying opposite poles—is a vital milestone during human embryo implantation. Although essential to embryogenesis and pregnancy, amniotic sac development in humans remains poorly understood. Here, we report a human pluripotent stem cell (hPSC)-based model, termed the post-implantation amniotic sac embryoid (PASE), that recapitulates multiple post-implantation embryogenic events centered around amniotic sac development. Without maternal or extraembryonic tissues, the PASE self-organizes into an epithelial cyst with an asymmetric amniotic ectoderm-epiblast pattern that resembles the human amniotic sac. Upon further development, the PASE initiates a process that resembles posterior primitive streak development in a SNAI1-dependent manner. Furthermore, we observe asymmetric BMP-SMAD signaling concurrent with PASE development, and establish that BMP-SMAD activation/inhibition modulates stable PASE development. This study reveals a previously unrecognized fate potential of human pluripotent stem cells and provides a platform for advancing human embryology.

Date: 2017
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DOI: 10.1038/s41467-017-00236-w

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