Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

Sebastiaan J. Trietsch, Elena Naumovska, Dorota Kurek, Meily C. Setyawati, Marianne K. Vormann, Karlijn J. Wilschut, Henriëtte L. Lanz, Arnaud Nicolas, Chee Ping Ng, Jos Joore, Stefan Kustermann, Adrian Roth, Thomas Hankemeier, Annie Moisan and Paul Vulto ()
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Sebastiaan J. Trietsch: Mimetas BV
Elena Naumovska: Mimetas BV
Dorota Kurek: Mimetas BV
Meily C. Setyawati: Mimetas BV
Marianne K. Vormann: Mimetas BV
Karlijn J. Wilschut: Mimetas BV
Henriëtte L. Lanz: Mimetas BV
Arnaud Nicolas: Mimetas BV
Chee Ping Ng: Mimetas BV
Jos Joore: Mimetas BV
Stefan Kustermann: Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd
Adrian Roth: Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd
Thomas Hankemeier: Leiden University
Annie Moisan: Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd
Paul Vulto: Mimetas BV

Nature Communications, 2017, vol. 8, issue 1, 1-8

Abstract: In vitro models that better reflect in vivo epithelial barrier (patho-)physiology are urgently required to predict adverse drug effects. Here we introduce extracellular matrix-supported intestinal tubules in perfused microfluidic devices, exhibiting tissue polarization and transporter expression. Forty leak-tight tubules are cultured in parallel on a single plate and their response to pharmacological stimuli is recorded over 125 h using automated imaging techniques. A study comprising 357 gut tubes is performed, of which 93% are leak tight before exposure. EC50-time curves could be extracted that provide insight into both concentration and exposure time response. Full compatibility with standard equipment and user-friendly operation make this Organ-on-a-Chip platform readily applicable in routine laboratories.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00259-3

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DOI: 10.1038/s41467-017-00259-3

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