Landscape of submitochondrial protein distribution

F.-Nora Vögtle, Julia M. Burkhart, Humberto Gonczarowska-Jorge, Cansu Kücükköse, Asli Aras Taskin, Dominik Kopczynski, Robert Ahrends, Dirk Mossmann, Albert Sickmann, René P. Zahedi () and Chris Meisinger ()
Additional contact information
F.-Nora Vögtle: University of Freiburg
Julia M. Burkhart: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
Humberto Gonczarowska-Jorge: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
Cansu Kücükköse: University of Freiburg
Asli Aras Taskin: University of Freiburg
Dominik Kopczynski: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
Robert Ahrends: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
Dirk Mossmann: University of Freiburg
Albert Sickmann: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
René P. Zahedi: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V
Chris Meisinger: University of Freiburg

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract The mitochondrial proteome comprises ~1000 (yeast)–1500 (human) different proteins, which are distributed into four different subcompartments. The sublocalization of these proteins within the organelle in most cases remains poorly defined. Here we describe an integrated approach combining stable isotope labeling, various protein enrichment and extraction strategies and quantitative mass spectrometry to produce a quantitative map of submitochondrial protein distribution in S. cerevisiae. This quantitative landscape enables a proteome-wide classification of 986 proteins into soluble, peripheral, and integral mitochondrial membrane proteins, and the assignment of 818 proteins into the four subcompartments: outer membrane, inner membrane, intermembrane space, or matrix. We also identified 206 proteins that were not previously annotated as localized to mitochondria. Furthermore, the protease Prd1, misannotated as intermembrane space protein, could be re-assigned and characterized as a presequence peptide degrading enzyme in the matrix.

Date: 2017
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DOI: 10.1038/s41467-017-00359-0

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