Biochemical phosphates observed using hyperpolarized 31P in physiological aqueous solutions

Atara Nardi-Schreiber, Ayelet Gamliel, Talia Harris, Gal Sapir, Jacob Sosna, J. Moshe Gomori and Rachel Katz-Brull ()
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Atara Nardi-Schreiber: Hadassah-Hebrew University Medical Center
Ayelet Gamliel: Hadassah-Hebrew University Medical Center
Talia Harris: Hadassah-Hebrew University Medical Center
Gal Sapir: Hadassah-Hebrew University Medical Center
Jacob Sosna: Hadassah-Hebrew University Medical Center
J. Moshe Gomori: Hadassah-Hebrew University Medical Center
Rachel Katz-Brull: Hadassah-Hebrew University Medical Center

Nature Communications, 2017, vol. 8, issue 1, 1-7

Abstract: Abstract The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of 31P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in D2O (T1 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in a cell-free system via the chemical shift of hyperpolarized inorganic phosphate. Because the 31P nucleus is 100% naturally abundant, future studies on hyperpolarized phosphates will not require expensive isotope labeling as is usually required for hyperpolarization of other substrates.

Date: 2017
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DOI: 10.1038/s41467-017-00364-3

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