P-body proteins regulate transcriptional rewiring to promote DNA replication stress resistance

Raphael Loll-Krippleber and Grant W. Brown ()
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Raphael Loll-Krippleber: University of Toronto
Grant W. Brown: University of Toronto

Nature Communications, 2017, vol. 8, issue 1, 1-15

Abstract: Abstract mRNA-processing (P-) bodies are cytoplasmic granules that form in eukaryotic cells in response to numerous stresses to serve as sites of degradation and storage of mRNAs. Functional P-bodies are critical for the DNA replication stress response in yeast, yet the repertoire of P-body targets and the mechanisms by which P-bodies promote replication stress resistance are unknown. In this study we identify the complete complement of mRNA targets of P-bodies during replication stress induced by hydroxyurea treatment. The key P-body protein Lsm1 controls the abundance of HHT1, ACF4, ARL3, TMA16, RRS1 and YOX1 mRNAs to prevent their toxic accumulation during replication stress. Accumulation of YOX1 mRNA causes aberrant downregulation of a network of genes critical for DNA replication stress resistance and leads to toxic acetaldehyde accumulation. Our data reveal the scope and the targets of regulation by P-body proteins during the DNA replication stress response.

Date: 2017
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DOI: 10.1038/s41467-017-00632-2

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