Functional organization of cytoplasmic inclusion bodies in cells infected by respiratory syncytial virus

Vincent Rincheval, Mickael Lelek, Elyanne Gault, Camille Bouillier, Delphine Sitterlin, Sabine Blouquit-Laye, Marie Galloux, Christophe Zimmer, Jean-François Eleouet and Marie-Anne Rameix-Welti ()
Additional contact information
Vincent Rincheval: Université de Versailles St. Quentin
Mickael Lelek: Institut Pasteur Unité Imagerie et Modélisation
Elyanne Gault: Université de Versailles St. Quentin
Camille Bouillier: Université de Versailles St. Quentin
Delphine Sitterlin: Université de Versailles St. Quentin
Sabine Blouquit-Laye: Université de Versailles St. Quentin
Marie Galloux: Université Paris-Saclay
Christophe Zimmer: Institut Pasteur Unité Imagerie et Modélisation
Jean-François Eleouet: Université Paris-Saclay
Marie-Anne Rameix-Welti: Université de Versailles St. Quentin

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Infection of cells by respiratory syncytial virus induces the formation of cytoplasmic inclusion bodies (IBs) where all the components of the viral RNA polymerase complex are concentrated. However, the exact organization and function of these IBs remain unclear. In this study, we use conventional and super-resolution imaging to dissect the internal structure of IBs. We observe that newly synthetized viral mRNA and the viral transcription anti-terminator M2-1 concentrate in IB sub-compartments, which we term “IB-associated granules” (IBAGs). In contrast, viral genomic RNA, the nucleoprotein, the L polymerase and its cofactor P are excluded from IBAGs. Live imaging reveals that IBAGs are highly dynamic structures. Our data show that IBs are the main site of viral RNA synthesis. They further suggest that shortly after synthesis in IBs, viral mRNAs and M2-1 transiently concentrate in IBAGs before reaching the cytosol and suggest a novel post-transcriptional function for M2-1.

Date: 2017
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/s41467-017-00655-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00655-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-00655-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().