The catalytic subunit of DNA polymerase δ inhibits γTuRC activity and regulates Golgi-derived microtubules

Yuehong Shen, Pengfei Liu, Taolue Jiang, Yu Hu, Franco K. C. Au and Robert Z. Qi ()
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Yuehong Shen: The Hong Kong University of Science and Technology
Pengfei Liu: The Hong Kong University of Science and Technology
Taolue Jiang: The Hong Kong University of Science and Technology
Yu Hu: The Hong Kong University of Science and Technology
Franco K. C. Au: The Hong Kong University of Science and Technology
Robert Z. Qi: The Hong Kong University of Science and Technology

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: γ-Tubulin ring complexes (γTuRCs) initiate microtubule growth and mediate microtubule attachment at microtubule-organizing centers, such as centrosomes and the Golgi complex. However, the mechanisms that control γTuRC-mediated microtubule nucleation have remained mostly unknown. Here, we show that the DNA polymerase δ catalytic subunit (PolD1) binds directly to γTuRCs and potently inhibits γTuRC-mediated microtubule nucleation. Whereas PolD1 depletion through RNA interference does not influence centrosome-based microtubule growth, the depletion augments microtubule nucleation at the Golgi complex. Conversely, PolD1 overexpression inhibits Golgi-based microtubule nucleation. Golgi-derived microtubules are required for the assembly and maintenance of the proper Golgi structure, and we found that alteration of PolD1 levels affects Golgi structural organization. Moreover, suppression of PolD1 expression impairs Golgi reassembly after nocodazole-induced disassembly and causes defects in Golgi reorientation and directional cell migration. Collectively, these results reveal a mechanism that controls noncentrosomal γTuRC activity and regulates the organization of Golgi-derived microtubules.

Date: 2017
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DOI: 10.1038/s41467-017-00694-2

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