Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis

Yan Liu, Michael J. Conboy, Melod Mehdipour, Yutong Liu, Thanhtra P. Tran, Aaron Blotnick, Prasanna Rajan, Thalie Cavalcante Santos and Irina M. Conboy ()
Additional contact information
Yan Liu: UC Berkeley and QB3 Institutes
Michael J. Conboy: UC Berkeley and QB3 Institutes
Melod Mehdipour: UC Berkeley and QB3 Institutes
Yutong Liu: UC Berkeley and QB3 Institutes
Thanhtra P. Tran: UC Berkeley and QB3 Institutes
Aaron Blotnick: UC Berkeley and QB3 Institutes
Prasanna Rajan: UC Berkeley and QB3 Institutes
Thalie Cavalcante Santos: UC Berkeley and QB3 Institutes
Irina M. Conboy: UC Berkeley and QB3 Institutes

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal proteome labeling for detecting in vivo proteins present only in transplanted myoblasts, but not in host tissue, and proteins exclusive to one young mouse and transferred during parabiosis to its old partner. We use a transgenic mouse strain that ubiquitously expresses a modified tRNA methionine synthase, metRS, which preferentially incorporates the methionine surrogate azido-nor-leucine (ANL) into newly generated proteins. Using click chemistry and a modified antibody array to detect ANL-labeled proteins, we identify several ‘young’ systemic factors in old regenerating muscle of the heterochronic parabiotic partners. Our approach enables the selective profiling of mammalian proteomes in mixed biological environments such as cell and tissue transplantation, apheresis or parabiosis.

Date: 2017
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-017-00698-y Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00698-y

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-00698-y

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().